Abstract the leaf essential oils of seven ocimum basilicum l. Brine shrimp lethality bioassay of selected indian medicinal. The proteins from foot and digestive gland were processed and their concentration determined by microtitre technique. Assessment of bioactivity of indian medicinal plants using brine shrimp artemia salina lethality assay.
Uji toksisitas dengan menggunakan metode brine shrimp lethality test bslt pada ekstrak mangrove avicennia marina, rhizophora mucronata, sonneratia alba dan xylocarpus granatum yang berasal. Open access a comparison between two brine shrimp assays to. The yellow fever mosquito larvae lethality test a bioassay for pesticides. Known toxicity properties based on the number of larvae mortality. Cyclophosphamide was used a standard anticancer test drug. Brine shrimp lethality test and acetylcholine esterase inhibition studies on selected south african medicinal plants.
Brine shrimp lethality bioassay is a simple, high throughput cytotoxicity test of bioactive chemicals. Pharm, department of pharmacy faculty of science and engineering. An application of the brine shrimp bioassay for general. It has also been suggested for screening pharmacological activities in plant extracts. Brine shrimp lethality assay is an essential tool used for preliminary assessment of toxicity of medicinal plant products by measuring their.
The brine shrimp lethality assay bsla has been used routinely in the primary screening of the crude extracts as well as isolated compounds to assess the toxicity towards brine shrimp. Solutions of the extracts were made in dmso or distilled water at varying concentrations and were incubated in triplicate vials with the brine shrimp larvae. The brine shrimp lethality bioassay is a rapid and inexpensive test requiring a relatively small amount of sample 220 mg. The study tested for in vivo brine shrimp lethality test bslt of soluble protein extracts from biomphalaria pfeifferi snail. Englerin, a selective anticancer drug against kidney cancer cells was isolated from phyllanthes engleri, predicted by. The method is attractive, because it is very simple, inexpensive and sensitive 8. Performed by mohammad shah hafez kabir founder and ceo, gusto a research group chief trainer at sage research training centre b. A small tank was filled with filtered sterile seawater that was divided into two compartments. A cytotoxic activity of tahongai kleinhovia hospita linn. P brine shrimp lethality assay and ld 50 oftheacute oral toxicity assay in mice. The brine shrimp assay has advantages of being rapid 24. Pdf uji toksisitas dengan menggunakan metode brine shrimp.
An application of the brine shrimp bioassay for general screening. Antidiarrheal activity and brine shrimp lethality bioassay. Antimicrobial activity and brine shrimp lethality bioassay of the. The hatching chamber was covered with black material and the other half of the tank was lighted to attract and separate the hatched shrimp. Assay procedures and analysis of results was done as reported earlier moshi and mbwambo, 2005. Toxicities of compounds were tested at 50, 100, 300, 800 and ppm in 10 ml seawater solutions with 1% dmso vv. Brine shrimp lethality and antioxidant activity of the leaf. The medium in which the test was conducted was artificial sea water, made by dissolving 38 g of crude salt in 1 l of distilled water.
Englerin, a selective anticancer drug against kidney cancer cells was isolated from phyllanthes engleri, predicted by brine shrimp test 15. Brine shrimp lethality assay the brine shrimp lethality assay was carried out with slight modification. Toxicity test with brine shrimp lethality test bst method using artemia salina was conducted to find out toxic effect of g. Brine shrimp lethality assay bsla the brine shrimp eggs were provided by the chemistry department of msuiit. Brine shrimp lethality assay of the aqueous and ethanolic. Brine shrimp lethality assay is an important tool for the preliminary cytotoxicity assay of plant extract and others based on the ability to kill a. Phytochemical analysis, antioxidant properties and brine shrimp. Pdf brine shrimp lethality test of soluble proteis from. Brine shrimp lethality test bslt method has been used as preliminary test for screening the activity of chemical compounds in n hexane, ethyl acetate, and methanol extracts from mesocarp and seeds of phaleria macrocarpa, fam. Sep 23, 2002 the brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. Statistical analysis with two way anova showed extract and concentration had a significant p lethality percentage. Novel cytotoxic, antitumor, and pesticidal compounds can be isolated from potential plant sources through the assessment of cytotoxic. The brine shrimp lethality test bst was used to predict the presence, in the extracts, of cytotoxic activity.
Brine shrimp lethality assay bangladesh journals online. Pdf brine shrimp lethality assay of whole plant extracts of. Brine shrimp lethality test the extracts, fractions and pure isolated compounds were routinely evaluated in a test for lethality to brine shrimp larvae 3, with minor modifications. Brine shrimp lethality bioassay brine shrimp lethality bioassay is considered a useful tool for preliminary assessment of toxicity. Cytotoxicity test brine shrimp lethality bioassay with lc50. Artemia salina, brazilian plants, brine shrimp, cytotoxicity. Brine shrimps were hatched using brine shrimp eggs in a small glass aquarium filled with seawater ph8. The brine shrimp lethality test the brine shrimp lethality test bst was used to predict the presence, in the extracts, of cytotoxic activity meyer et al. Pdf brine shrimp lethality assay of whole plant extracts. The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. These two bioassays show that standard tests employing organisms such as brine shrimp are useful in identifying metabolites with a high potential for activity against marine organisms.
Brine shrimp lethality test bslt merupakan suatu metoda yang digunakan untuk menguji bahanbahan yang bersifat toksik ahmed et al. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Toxicity and phytochemistry test of methanol extract of. Filtered, artificial seawater was prepared by dissolving 38 g of sea salt in 1 liter of distilled water for hatching the shrimp eggs.
Biosatellite ii, apollo 16, and apollo 17 missions, and on the russian bion3, bion5 cosmos 1129, foton 10, and foton 11 flights. Brine shrimp lethality bioassay is a simple, high throughput cytotoxicity test of. A thesis submitted in partial fulfilment of the requirements. Piper longum showed most potent cytotoxic activity. Sep 30, 2017 what is brine shrimp lethality assay cytotoxicity test, protocol, the procedure, kit of protocol advantages and disadvantages. It is based on the killing ability of test compounds on a simple zoological organismbrine shrimp artemia salina.
Brine shrimp test the bioactivity of the extracts was monitored by the brine shrimp lethality test 7 to predict the cytotoxic activity in the compound. Sea water was kept in a glass trough and one spoon of cyst shrimp eggs was put into the trough, properly sealed with aluminium foil and few pores were made for constant air supply and maintained at 37 c. Chemical composition of essential oil from seven ocimum. On test day the extracts were dissolved in dmso to appropriate working concentrations. The brine shrimp lethality of the two test samples both for decoction and ethanolic extract were found to be concentrationdependent as shown in table 1. Piperine, guggulsterone e and guggulsterone z showed potent activity with lc 50 2. The cysts hatch to nauplii when deposited in saline seawater. Assessment of bioactivity of indian medicinal plants using. Brine shrimp lethality assay of the ethanolic extracts of. Brine shrimp eggs, artemia salina were hatched in artificial sea water prepared by dissolving 38 g. A simple test which has been widely used as an indication of cytotoxicity is based on lethality to the larvae of the brine shrimp artemia salina 3942.
Toxiclogical evaluation of the plant products using brine shrimp. However, we think that it is necessary to evaluate the suitability of the brine shrimp methods before they are used as a general bioassay to test natural marine products for pharmacological activity. A comparison between two brine shrimp assays to detect in vitro. Brine shrimp lethality test the brine shrimp lethality test bst was used to assay cytotoxic activity meyer et al. Brine shrimp lethality bioassay tests to find out the presence of chemical that has a cytotoxic effects on cell line. Brine shrimp lethality assay is the most convenient system for. Brine shrimp lethality bioassay is a simple, rapid, inexpensive and high throughput cytotoxicity test of bioactive metabolites based on the killing ability of brine shrimp artemia salina. Evaluating the potential cytotoxic activity of ficus nota. Acute oral toxicity and brine shrimp lethality of methanol. An extract would have toxic effect if the lc 50 brine shrimp lethality test bslt brine shrimp lethality bioassay was carried out to investigate the toxicity effect of the three different brands of food seasonings in the philippines. The extracts was dissolved in methanol and diluted with sea water.
Thus, it is possible to detect and then monitor the fractionation of cytotoxic, as well as 3ps p388 in vivo murine leukaemia active extracts using the brine lethality bioassay alkofahi et al. Brine shrimp eggs were obtained from the new aqua laboratory in naawan, misamis oriental, as a gift sample for the research work. Brine shrimp lethality and acute oral toxicity of commiphora. Acute oral toxicity and brine shrimp lethality of pterocarpus. Brine shrimp lethality bioassay method was used to determine cytotoxic activity of cordyline fruticosa where brine shrimps artemia salina leach nauplii were used as test organisms.
Brine shrimp lethality test bslt is one of the methods bioactive compounds present in natural materials using shrimp larvae a. Brine shrimp lethality and acute oral toxicity studies on. The use of plants in various parts of the world for both preventive and curative purposes is an ancient tradition and is increasing pragmatically. Evaluation of cytotoxicity in selected species of caralluma. P brine shrimp lethality bioassay is a simple, high throughput cytotoxicity test of bioactive chemicals. Jan 20, 2017 performed by mohammad shah hafez kabir founder and ceo, gusto a research group chief trainer at sage research training centre b. Herbal science and medicine institute at the university. An important player in brine shrimp lethality bioassay.
Ethanolic extracts of six indian medicinal plants, piperine, guggulsterone e and guggulsterone z were tested for cytotoxicity using brine shrimp lethality test. For the experiment, 32 mg of test sample was dissolved in dimethyl sulfoxide dmso and solutions of varying concentrations 800. The test groups were divided into k0, k1, k2, and k3 groups. Jun 05, 2017 brine shrimp lethality bioassay is a simple, rapid, inexpensive and high throughput cytotoxicity test of bioactive metabolites based on the killing ability of brine shrimp artemia salina. Brine shrimp lethality test and acetylcholine esterase. Ganoderma lucidum is polyporus fungi from basidiomycetes which can be used as traditional medicines. Both the decoction and ethanolic extracts showed direct relationship with the mortality rate of the brine shrimp. This confirmed that the brine shrimp lethality assay was useful for the screening of the plant extract, to predict the toxicity level. The inhibition of crown gall tumors on discs of potato tubers an antitumor bioassay, 3. Kajian toksisitas dari tubuh buah ganoderma lucidum dengan.
The results demonstrated that the yield of tannins for the acetone. Toksisitas didefinisikan sebagai kemampuan suatu zat yang memiliki sifat destruktif pada sel terutama yang menyangkut proses suatu sel dalam sistem kekebalan tubuh clayman, 1989. Brine shrimp lethality assay bsla is an important method in the evaluation of plantbioactivity for the subsequent isolation of bioactive compounds from the plant extracts which may lead tothe development of new drugs. The present study was conducted to test for in vivo brine shrimp lethality assay bsla of the ethanolic extracts of lantana camara, chromolaena odorata, and euphorbia hirta and correlate cytotoxicity results with known pharmacological activities of the plants. It is based on the killing ability of test compounds on a simple zoological organism brine shrimp artemia salina. Scientists have taken the eggs of brine shrimp to outer space to test the impact of radiation on life. They were tested for cytotoxicity using brine shrimp lethality test and for their ability to inhibit the enzymatic activity of the glycossomal glyceraldehyde3phosphate dehydrogenase gapdh from trypanosoma cruzi and adenine phosphoribosyltransferase aprt from leishmania. Besides, the fractions were screened for cytotoxic activity using brine shrimp artemia salina lethality bioassay. Lagarto, demonstrated that there was a good correlationr 0. Both the decoction and ethanolic extracts showed direct relationship with the mortality rate of the brine shrimp because as the concentration of the ficus nota. Some of the russian flights carried european space agency experiments.
The study aimed to determine cytotoxicity assay on collagenchitosan wound dressing sterilized by ultraviolet uv light using brine shrimp lethality test bslt method. The inhibition of frond proliferation in duckweed a bioassay for herbicides and plant growth stimulants, and 4. Antimicrobial activity and brine shrimp toxicity of extracts. Brine shrimp lethality test bslt dari berbagai fraksi. In the brine shrimp lethality bioassay, the lc 50 and lc 90 of a. In order to achieve a good correlation between pediculicidal activity and brine shrimp lethality bsl test, several pediculicidal substances and active essential. Assay procedures and analysis of results was done as reported earlier. Brine shrimp lethality test bst bst was done as a monitor for bioactivity and as a predictor for the presence of cytotoxic activity in the exudate.
904 490 1426 387 871 96 1088 1159 567 1208 1452 1172 958 188 1076 150 1174 1291 470 895 557 953 324 743 1394 195 128 1232 843 840 1034 1095 246 1216 536 22 697 904 1072 348 82 627 1105 297 1281 974